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    Dojindo Labs dojindo cat 341 07761
    Dojindo Cat 341 07761, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 59887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dojindo+cat/Cell+Counting+Kit-8/pmc13049664-165-12-12
    Average 99 stars, based on 59887 article reviews
    dojindo cat 341 07761 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Single Cell:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    CCK-8 Assay:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    Article Title: Ectopic CD11c Drives SMAD3-Mediated Aberrant Antigen Presentation and Epithelial–Mesenchymal Transition in Esophageal Squamous Cell Carcinoma
    Article Snippet: .. We seeded ESCC cells in 96-well plates and assessed cell viability at 5 time points using Cell Counting Kit-8 (DOJINDO, Cat. #CK04). ..

    Article Title: Extracellular matrix-growth factor signalling drives the oncogenic mir-125b-2/UCK2 axis in hepatocellular carcinoma.
    Article Snippet: After washing, membranes were probed with HRP-conjugated secondary antibodies, goat anti-rabbit IgG (Thermo Scientific, Cat. #31460) or goat antimouse IgG (Thermo Scientific, Cat. #31430), and visualized using SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, Cat. #34580). .. Proliferation was evaluated 24–72 h after transfection using the Cell Counting Kit-8 (CCK-8; Dojindo, Cat. #CK04). ..

    Article Title: Transcriptional activation of PPP1R14C by KLF7 unleashes CDK1 activity to promote lung squamous cell carcinoma.
    Article Snippet: .. For proliferation, 2×103 cells/well were seeded in 96-well plates, and absorbance was measured at 0, 24, 48, 72, and 96 h using Cell Counting Kit-8 (Dojindo, Cat. #CK04). ..

    Article Title: SHLP2 alleviates allergic asthma by inhibiting ETV5/GSDMD signaling pathway-mediated pyroptosis.
    Article Snippet: Background: Allergic asthma (AA) is a lung disease characterized by impaired respiratory function and significant infiltration of inflammatory cells.. Human Protein-Mimicking Peptide-2 (SHLP2) is a recently discovered mitochondria-encoded peptide that plays a crucial role in mitochondrial retrograde signaling pathways.. However, its potential utility in mitigating AA remains unexplored.

    Article Title: Transcriptional activation of PPP1R14C by KLF7 unleashes CDK1 activity to promote lung squamous cell carcinoma
    Article Snippet: .. For proliferation, 2 × 10 3 cells/well were seeded in 96-well plates, and absorbance was measured at 0, 24, 48, 72, and 96 h using Cell Counting Kit-8 (Dojindo, Cat. #CK04). ..

    Article Title: Salvia miltiorrhiza -derived microRNA58 inhibits tumor growth by targeting Krüppel-like factor 3 in human endothelial cells
    Article Snippet: .. Using a Cell Counting Kit-8 (CCK-8; Dojindo, cat. No. C0038; Kumamoto, Japan), the proliferation rate of the HUVECs was assessed. ..

    Sequencing:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    Whole Complete genome sequencing:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    Biomarker Discovery:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    RNA Sequencing:

    Article Title: Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triton X-100 Sangon Biotech Cat#A600198 DAPI Beyotime Cat#C1002 Trypan blue Thermo Fisher Scientific Cat#15250061 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen Cat#AM9260G Bovine Serum Albumin (BSA) Sangon Biotech Cat#A500023-0100 Nylon cell strainer (40μm) Corning Cat#352340 Glass bottom culture dishes NEST Cat#801001 Matrigel Corning Cat#356231 100mm Culture Dishes Corning Cat#430167 60mm Culture Dishes Corning Cat#430166 6-well plates Corning Cat#3516 96-well plates Corning Cat#3798 U-bottom 96-well plates Corning Cat#7007 anti-human PD-1 monoclonal antibody Shlleck Cat#A2002 ARPN2169 APEIRON This study FACS Staining Buffer Invitrogen Cat#00-4222-26 Blocking buffer Invitrogen Cat#B001T06F01 Cell Activation Cocktail BioLegend Cat#423303 GlutaMAX TM supplement Gibco Cat#35050061 N-2 supplement Gibco Cat#17502048 MEM Non-Essential Amino Acids Gibco Cat#11140050 B-27 TM supplement Gibco Cat#17504044 Insulin, Transferrin, Selenium Solution (ITS-G) Gibco Cat#41400045 Ascorbic acid Merck Cat#200-066-2 ROCK inhibitor Y-27632 Merck Cat#10035-04-8 Human recombinant EGF protein PeproTech Cat#AF-100-15 Human recombinant bFGF protein MedChemExpress Cat#HY-P7331A Human recombinant IGF-1 protein MedChemExpress Cat#HY-P70783 Human recombinant TGF-β3 protein MedChemExpress Cat#HY-P7120 Adenine MedChemExpress Cat#HY-B0152 ALK inhibitor A 83-01 MedChemExpress Cat#HY-10432 p38 MAPK inhibitor SB 202190 MedChemExpress Cat#HY-10295 Dexamethasone MedChemExpress Cat#HY-14648 Human recombinant Wnt3a protein MedChemExpress Cat#HY-P70453A Serum-free cell cryopreservation solution NCM Biotech Cat#C40100 Anti-human CD3 antibody BioLegend Cat#300306 CD28 Monoclonal Antibody Invitrogen Cat#16-0289-85 Human Recombinant IL-2 PeproTech Cat#200-02 Human IgG1 isotype control Shlleck Cat#A2051 Doxorubicin hydrochloride MedChemExpress Cat#HY-15142 Agarose Servicebio Cat#G5056-5G CellTiter-Lumi TM Luminescent Cell Viability Assay Kit Beyotime Cat#C0065S Nuclease-free ATP Beyotime Cat#D7378 CTAB lysis buffer Promega Cat#MC1411 TE buffer Thermo Fisher Scientific Cat#12090015 Agencourt AMPure XP-Medium kit Beckman Cat#A63880 (Continued on next page) e2 Cell Reports Medicine 7, 102644, March 17, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#PN-1000268 RNeasy Mini Kit QIAGEN Cat#74104 SeekOne DD Single Cell 3 ′ Transcriptome kit SeekGene N/A RNA Nano 6000 Assay Kit Agilent Cat#5067-1511 NEBNext® Ultra TM RNA Library Prep Kit for Illumina® NEB Cat#E7530 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat#Q32851 Cell Counting Kit-8 Dojindo CatCK04 Deposited data Whole genome sequencing (WGS) data of iOS National Genomics Data Center (This study) HRA008992 SGH-OS multi-omics dataset National Genomics Data Center HRA003260 TCGA-SARC dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ TARGET-OS dataset Genomic Data Commons Data Protal https://portal.gdc.cancer.gov/ Single-cell RNA-seq of Osteosarcoma National Genomics Data Center - OMIX (This study) OMIX013436 Experimental models: Cell lines DuNN Dunn Lab RRID: CVCL_W629 DuNN MTAP − /− Shanghai General Hospital N/A ..

    Transfection:

    Article Title: Extracellular matrix-growth factor signalling drives the oncogenic mir-125b-2/UCK2 axis in hepatocellular carcinoma.
    Article Snippet: After washing, membranes were probed with HRP-conjugated secondary antibodies, goat anti-rabbit IgG (Thermo Scientific, Cat. #31460) or goat antimouse IgG (Thermo Scientific, Cat. #31430), and visualized using SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, Cat. #34580). .. Proliferation was evaluated 24–72 h after transfection using the Cell Counting Kit-8 (CCK-8; Dojindo, Cat. #CK04). ..



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